ABSTRACT
Objective To investigate the expression of Parkin protein in colorectal cancer and its clinical significance. Methods Immunohistochemistry was used to detect the expression of Parkin protein in normal and cancerous tissues of 50 patients with colorectal cancer from 2012 to 2015. The relationship between clinicopathological characteristics and Parkin protein expression was analyzed. Results The positive expression rate of Parkin protein in cancerous tissues was significantly lower than that in normal tissues: 36% (18/50) vs. 72% (36/50), and there was statistical difference (P＜0.01). The positive expression of Parkin protein was correlated with the degree of tissue differentiation (P < 0.01);but it was not correlated with sex, age, tumor diameter, tumor type, tumor infiltration, lymph node metastasis and clinical stage (P＞0.05). Conclusions In colorectal cancer tissues, the expression of Parkin gene is absent and decreased. The abnormal expression of Parkin gene may be related to the occurrence and development of colorectal cancer.
ABSTRACT
Objective To investigate the relationship between single nucleotide polymorphism in estrogen metabolizing genes CYP17、CYP19 and breast cancer susceptibility.Methods A case-control study was performed.PCR-base restriction fragment length polymorphism(PCR-RFLP)and short tandem repeat polymorphism(STRP)assay were used to detect the single nucleotide polymorphism of CYP17、CYP19 in 213 breast cancer cases and 430 matched controls.Resuits CYP17 A2/A2 genotype was found in 6.7%of breast cancer cases,which was significantly higher(P＜0.05)than that in controls(2.4%);the frequency of A2 allele of CYP17 was 16.2%in breast cancer cases,which was significantly higher(P＜0.05)than that in controls(10.6%).There Was alSO significant difference in the frequency of(TTTA)10allele of CYP19 which was 12.4%in breast cancer cases and 8.2%in controls(P=0.02).Conclusions The allele of CYP17 A2 and CYP19(TTTA)10 and CYP17 A2/A2 genotype were positively associated with the susceptibility of breast cancer.